j774 a 1 cells  (ATCC)

Journal: Science Advances

Article Title: DksA inhibitors against intracellular and persistent Salmonella are effective in acute models of infection

doi: 10.1126/sciadv.aea6832

Figure Lengend Snippet: ( A ) Intracellular replication of the indicated Salmonella strains 20 hours after infection of J774 cells with an MOI of 2. Where indicated, J774 cells were treated with compound 916. *** P < 0.001 as determined by one-way analysis of variance (ANOVA). ( B ) Intracellular SPI-2 gene transcription was assessed measure luminescence signal from To and T8 treated with drug #916 (16 μg/ml) or without. Data are the mean ± SD from two independent experiments, P < 0.001 by one-way ANOVA.

Article Snippet: Macrophage-like J774 murine cells (ATCC TIB-67) were maintained in RPMI + media [RPMI media supplemented with 2 mM l -glutamate, 1.0 mM sodium pyruvate, 15 mM Hepes buffer, 10% fetal bovine serum, and penicillin-streptomycin (100 U/ml)] in a 5% CO 2 incubator at 37°C.

Techniques: Infection

Journal: Science Advances

Article Title: DksA inhibitors against intracellular and persistent Salmonella are effective in acute models of infection

doi: 10.1126/sciadv.aea6832

Figure Lengend Snippet: ( A ) The MIC of compounds VKT-17-P4-21 (#21) and VKT-17-P4-23 (#23) was determined against several Gram-negative pathogens isolated at the Microbiology Clinical Laboratory at the University of Colorado Hospital. ( B ) Antimicrobial activity of anti-DksA drugs against intracellular Salmonella after 20 hours of culture in J774 cells. Data are the mean ± SD from three to four independent experiments. Four micrograms per milliliter is the minimal concentration of VKT-17-P4-21 and VKT-17-P4-23 at which there is statistical significance ( P < 0.0001 by two-way ANOVA) when compared to untreated controls.

Article Snippet: Macrophage-like J774 murine cells (ATCC TIB-67) were maintained in RPMI + media [RPMI media supplemented with 2 mM l -glutamate, 1.0 mM sodium pyruvate, 15 mM Hepes buffer, 10% fetal bovine serum, and penicillin-streptomycin (100 U/ml)] in a 5% CO 2 incubator at 37°C.

Techniques: Isolation, Activity Assay, Concentration Assay